{"id":14136,"date":"2026-02-02T07:08:41","date_gmt":"2026-02-02T07:08:41","guid":{"rendered":"https:\/\/bio.unibuc.ro\/infrastructura-pcbe\/"},"modified":"2026-02-02T07:08:41","modified_gmt":"2026-02-02T07:08:41","slug":"infrastructura-pcbe","status":"publish","type":"post","link":"https:\/\/bio.unibuc.ro\/en\/infrastructura-pcbe\/","title":{"rendered":"Infrastructura PCBE"},"content":{"rendered":"

Platforma de Cercetare \u00een Biologie \u0219i Ecologie Sistemic\u0103 (PCBE)<\/strong>, apar\u021bin\u00e2nd Facult\u0103\u021bii de Biologie, Universitatea din Bucure\u0219ti<\/strong>, reune\u0219te pe mai multe etaje o infrastructur\u0103 modern\u0103 pentru cercetare fundamental\u0103 \u0219i aplicat\u0103 \u2013 de la biologie molecular\u0103 \u0219i genomic\u0103, p\u00e2n\u0103 la culturi celulare, imagistic\u0103 avansat\u0103, citometrie \u00een flux, secven\u021biere de nou\u0103 genera\u021bie \u0219i microbiologie. PCBE este g\u00e2ndit\u0103 ca un ecosistem integrat, care sus\u021bine \u00eentregul traseu experimental: preg\u0103tirea probelor \u2192 analize omice \u2192 validare molecular\u0103 \u2192 experimente celulare \u2192 imagistic\u0103 \u0219i fenotipare \u2192 secven\u021biere \u0219i analiz\u0103 microbian\u0103.<\/strong><\/p>\n

Etajul I \u2013 Analize de mediu, chimie analitic\u0103 \u0219i procese eco-tehnologice<\/h2>\n

Etajul I al Platformei de Cercetare \u00een Biologie \u0219i Ecologie Sistemic\u0103 (PCBE) este dedicat analizelor chimice avansate ale probelor de mediu<\/strong>, dezvolt\u0103rii de procese eco-tehnologice<\/strong> \u0219i caracteriz\u0103rii detaliate a compu\u0219ilor organici \u0219i anorganici din ecosisteme. Infrastructura sus\u021bine cercet\u0103ri interdisciplinare la interfa\u021ba dintre biologie, chimie \u0219i \u0219tiin\u021ba mediului, oferind un cadru experimental complet \u2013 de la preg\u0103tirea \u0219i extrac\u021bia probelor, p\u00e2n\u0103 la identificarea molecular\u0103 \u0219i evaluarea proceselor de remediere.<\/p>\n

Platforma include sisteme performante pentru analize elementare \u0219i izotopice<\/strong>, utilizate \u00een studiul re\u021belelor trofice, identificarea surselor de energie din ecosisteme \u0219i investigarea ciclurilor biogeochimice. Determinarea carbonului total, organic sau anorganic \u00een probe lichide \u0219i solide este realizat\u0103 prin analizatoare dedicate, esen\u021biale \u00een monitorizarea calit\u0103\u021bii apelor, solurilor \u0219i sedimentelor.<\/p>\n

Pentru caracterizarea contaminan\u021bilor \u0219i a biomoleculelor cu mas\u0103 molecular\u0103 mic\u0103, etajul dispune de o infrastructur\u0103 complex\u0103 de cromatografie de lichide \u0219i gaze<\/strong>, cuplat\u0103 cu detec\u021bie spectrometric\u0103 de mas\u0103 sau cu detectoare specifice (fluorescen\u021b\u0103, conductivitate, UV-DAD, RI). Aceste sisteme permit identificarea \u0219i cuantificarea unor compu\u0219i xenobiotici, precum pesticide sau produse farmaceutice, dar \u0219i analiza hidrocarburilor, glucidelor sau a compu\u0219ilor organici volatili \u0219i semivolatili. \u00cen completare, un sistem automatizat de gel-permea\u021bie<\/strong> faciliteaz\u0103 purificarea \u0219i concentrarea probelor \u00eenaintea analizelor cromatografice.<\/p>\n

Caracterizarea structural\u0103 \u0219i func\u021bional\u0103 a materialelor \u0219i probelor biologice este sus\u021binut\u0103 de tehnici spectroscopice moderne, precum spectroscopia FT-IR<\/strong> \u0219i spectrofotometria UV-VIS, inclusiv monitorizarea reac\u021biilor chimice sau enzimatice \u00een condi\u021bii controlate. Platforma include \u0219i solu\u021bii pentru extrac\u021bia accelerat\u0103 cu solven\u021bi<\/strong>, utile \u00een recuperarea compu\u0219ilor organici din matrici solide complexe.O component\u0103 distinctiv\u0103 a etajului este dedicat\u0103 dezvolt\u0103rii \u0219i test\u0103rii proceselor de tratare \u0219i epurare a apelor<\/strong>, incluz\u00e2nd instala\u021bii experimentale cu reactoare catalitice \u0219i sisteme modulare cu membrane, precum \u0219i echipamente pentru procese de oxidare avansat\u0103 pe baz\u0103 de ozon. Acestea permit simularea condi\u021biilor reale de remediere \u0219i optimizarea tehnologiilor de reducere a polu\u0103rii \u00een medii acvatice.<\/p>\n

\u00cen zona de biotehnologie \u0219i microbiologie aplicat\u0103, etajul este echipat cu bioreactoare de laborator<\/strong>, incubatoare, centrifuge \u0219i sisteme de control termic, care sus\u021bin cultiv\u0103ri microbiene \u0219i experimente la scar\u0103 mic\u0103 \u0219i medie. Analizele oxidative \u0219i studiile asupra speciilor reactive sunt facilitate de un chemiluminometru dedicat, iar investigarea proceselor celulare este completat\u0103 de microscopie de fluorescen\u021b\u0103.<\/p>\n

Activit\u0103\u021bile experimentale sunt sus\u021binute de o infrastructur\u0103 auxiliar\u0103 extins\u0103 (rotaevaporatoare, sisteme de ap\u0103 ultrapur\u0103, balan\u021be analitice, instala\u021bii de vid, omogenizare ultrasonic\u0103), precum \u0219i de echipamente de teren<\/strong> pentru monitorizarea ecosistemelor \u2013 de la determinarea indicelui suprafe\u021bei foliare \u0219i a parametrilor fizico-chimici ai apelor, p\u00e2n\u0103 la studii asupra planctonului, sedimentelor \u0219i ihtiofaunei. Astfel, etajul I conecteaz\u0103 \u00een mod direct cercetarea de laborator cu observa\u021biile \u0219i m\u0103sur\u0103torile realizate in situ<\/strong>, oferind o perspectiv\u0103 integrat\u0103 asupra func\u021bion\u0103rii sistemelor naturale.<\/p>\n

Mai multe detalii tehnice sunt disponibile aici<\/strong><\/span><\/a>.<\/p>\n

Etajul II \u2013 Genomic\u0103, transcriptomic\u0103 \u0219i platforme pentru analize moleculare<\/h2>\n

Etajul II g\u0103zduie\u0219te nucleul de biologie molecular\u0103 \u0219i analize omice<\/strong>, construit \u00een jurul unei camere curate BS2+ (ISO8)<\/strong> de aproximativ 55 m\u00b2<\/strong>, compartimentat\u0103 \u00een trei zone dedicate (extrac\u021bie acizi nucleici, preg\u0103tire mixuri \u0219i cuplare), pentru a asigura fluxuri de lucru riguroase \u0219i controlul contamin\u0103rilor. Spa\u021biul este echipat cu hote cu flux laminar<\/strong> de \u00eenalt\u0103 eficien\u021b\u0103 (H14), oferind condi\u021bii optime pentru lucrul cu ADN\/ARN \u0219i pentru preg\u0103tirea reac\u021biilor de amplificare.<\/p>\n

Capacitatea analitic\u0103 este completat\u0103 de echipamente pentru cuantificarea \u0219i controlul calit\u0103\u021bii<\/strong> acizilor nucleici (spectrofotometrie UV-VIS \u0219i analiz\u0103 de integritate), precum \u0219i de o flot\u0103 de termociclere \u0219i sisteme Real-Time PCR, care permit at\u00e2t experimente de rutin\u0103, c\u00e2t \u0219i analize cantitative sensibile \u0219i multiplex. Pentru separarea \u0219i documentarea acizilor nucleici, etajul include sisteme de electroforez\u0103<\/strong> \u0219i un analizor de imagini UV-VIS<\/strong> compatibil cu o gam\u0103 larg\u0103 de coloran\u021bi fluorescen\u021bi.<\/p>\n

\u00cen plus, infrastructura sus\u021bine cercetarea de v\u00e2rf prin tehnologie Single-Cell<\/strong> (izolare\/procesare a celulelor individuale, transcrip\u021bie invers\u0103 \u0219i preamplificare) \u0219i o platform\u0103 microarray<\/strong> pentru aplica\u021bii precum expresie genic\u0103, CGH, microARN, metilare ADN sau ChIP. Componenta de analiz\u0103 genetic\u0103 \u0219i secven\u021biere este sus\u021binut\u0103 de un analizor genetic capilar<\/strong> pentru secven\u021biere de fragmente \u0219i de un sistem de secven\u021biere Ion PGM<\/strong> (NGS), inclusiv pentru evalu\u0103ri metagenomice pe markerul 16S rRNA.<\/p>\n

Mai multe detalii tehnice sunt disponibile aici<\/strong><\/span><\/a>.<\/p>\n

Etajul III \u2013 Biologie celular\u0103, proteomic\u0103 \u0219i fenotipare avansat\u0103<\/h2>\n

Etajul III este dedicat cercet\u0103rii experimentale \u00een biologie celular\u0103<\/strong>, imagistic\u0103<\/strong> \u0219i fenotipare<\/strong>, oferind condi\u021bii de lucru conforme standardelor de biosiguran\u021b\u0103. Piesa central\u0103 este o arie de culturi celulare<\/strong> organizat\u0103 ca clean room BS2+ (ISO8)<\/strong>, cu dou\u0103 zone distincte: una pentru linii celulare<\/strong> \u0219i una pentru culturi primare<\/strong>. Pe o suprafa\u021b\u0103 de aproximativ 72 m\u00b2<\/strong>, infrastructura include hote de biosiguran\u021b\u0103 clasa II<\/strong>, incubatoare CO\u2082 \u0219i echipamente auxiliare pentru manipularea \u0219i men\u021binerea probelor biologice \u00een condi\u021bii controlate.<\/p>\n

Pentru analiza func\u021bional\u0103 \u0219i explorarea mecanismelor celulare, etajul dispune de un microscop confocal laser Nikon A1\/A1R<\/strong> (scanare point-scan, detector spectral multicanal), capabil s\u0103 livreze imagistic\u0103 de \u00eenalt\u0103 rezolu\u021bie \u0219i achizi\u021bii rapide \u2013 un instrument esen\u021bial \u00een studiile de localizare subcelular\u0103, colocalizare, dinamic\u0103 celular\u0103 \u0219i analize 3D. Pentru screening-uri \u0219i m\u0103sur\u0103tori spectrale, sunt disponibile sisteme UV\/VIS \u0219i cititoare multimod, utile at\u00e2t \u00een biologie celular\u0103, c\u00e2t \u0219i \u00een biochimie \u0219i test\u0103ri de interac\u021biuni moleculare.<\/p>\n

Etajul III sus\u021bine \u0219i fluxuri complete de electroforez\u0103 \u0219i Western blot<\/strong> (inclusiv transfer rapid), plus sisteme moderne de imagistic\u0103 pentru geluri \u0219i membrane (inclusiv detec\u021bie \u00een infraro\u0219u), oferind un cadru robust pentru valid\u0103ri proteice. Pentru preg\u0103tirea probelor biologice, sunt integrate solu\u021bii de sonicare<\/strong> (direct\u0103 \u0219i indirect\u0103), iar pentru studii pe termen lung \u00een condi\u021bii fiziologice, un microscop inversat automatizat permite live-cell imaging<\/strong> \u00een incubare CO\u2082. Complementar, un citometru \u00een flux cu mai multe lasere \u0219i detectoare ofer\u0103 analize multiparametrice<\/strong> pentru imunofenotipare, ciclul celular, viabilitate, expresie de markeri \u0219i caracterizare popula\u021bional\u0103.<\/p>\n

Mai multe detalii tehnice sunt disponibile aici<\/strong><\/span><\/a>.<\/p>\n

Etajul IV \u2013 Secven\u021biere NGS de ultim\u0103 genera\u021bie, bioprocesare \u0219i microbiologie<\/h2>\n

Etajul IV extinde capabilit\u0103\u021bile PCBE c\u0103tre genomic\u0103 la scar\u0103 mare<\/strong>, microbiologie<\/strong> \u0219i bioprocesare<\/strong>, fiind punctul de \u00eent\u00e2lnire dintre cercetarea fundamental\u0103 \u0219i aplica\u021biile cu relevan\u021b\u0103 biomedical\u0103 \u0219i de mediu. Componenta de secven\u021biere include o platform\u0103 Illumina MiSeq<\/strong>, adecvat\u0103 pentru ampliconi \u021binti\u021bi (ex. 16S\/ITS), genomuri mici bacteriene\/virale \u0219i metagenomic\u0103 de profunzime redus\u0103, precum \u0219i un sistem Oxford Nanopore MinION<\/strong> pentru citiri lungi, secven\u021biere \u00een timp real \u0219i aplica\u021bii precum metagenomic\u0103, plasmide, ampliconi sau secven\u021biere direct\u0103 de ARN.<\/p>\n

Pentru proiecte cu volum mare de date \u0219i aplica\u021bii omice complexe, etajul este echipat cu secven\u021biatoare PromethION<\/strong>, care ofer\u0103 capacitate ridicat\u0103 \u0219i flexibilitate pentru proiecte genomice, transcriptomice \u0219i epigenetice la scar\u0103 larg\u0103. \u00cen zona de bioprocesare, un bioreactor de banc de 4 L<\/strong> permite cultiv\u0103ri \u0219i fermenta\u021bii aerobe\/anaerobe cu monitorizare \u0219i control digital, sprijinind at\u00e2t optimiz\u0103ri de procese, c\u00e2t \u0219i studii experimentale asupra fiziologiei microbiene sau a interac\u021biunilor microb\u2013mediu.<\/p>\n

Etajul IV include \u0219i o platform\u0103 de microbiologie<\/strong> pentru activit\u0103\u021bi de cercetare cu microorganisme nepatogene, cu infrastructur\u0103 de baz\u0103 pentru cultivare, conservare \u0219i analiz\u0103 (incubatoare, ap\u0103 ultrapur\u0103, hote cu flux laminar, spectrofotometrie, centrifugare, microscopie). Fenotiparea celular\u0103 este completat\u0103 de un citometru \u00een flux compact, u\u0219or de utilizat, potrivit at\u00e2t pentru activit\u0103\u021bi de cercetare, c\u00e2t \u0219i pentru instruire avansat\u0103 \u0219i proiecte interdisciplinare.<\/p>\n

Mai multe detalii tehnice sunt disponibile aici<\/strong><\/span><\/a>.<\/p>\n

Un spa\u021biu deschis pentru formare, excelen\u021b\u0103 \u0219i colaborare<\/h2>\n

Prin infrastructura sa, PCBE sus\u021bine:<\/p>\n

    \n
  • \n

    preg\u0103tirea \u0219i formarea studen\u021bilor<\/strong> (licen\u021b\u0103, master, doctorat) \u00een tehnici moderne de laborator \u0219i metodologii omice;<\/p>\n<\/li>\n

  • \n

    proiecte de cercetare<\/strong> competitive, de la mecanisme moleculare la ecologie microbian\u0103 \u0219i biologie celular\u0103;<\/p>\n<\/li>\n

  • \n

    colabor\u0103ri<\/strong> cu institute \u0219i universit\u0103\u021bi, precum \u0219i proiecte aplicative cu parteneri din domeniul s\u0103n\u0103t\u0103\u021bii, biotehnologiei \u0219i mediului.<\/p>\n<\/li>\n<\/ul>\n

    PCBE este conceput\u0103 ca o platform\u0103 modular\u0103 \u0219i scalabil\u0103<\/strong>, \u00een care competen\u021bele \u0219i echipamentele se conecteaz\u0103 natural \u00eentre etaje, acceler\u00e2nd tranzi\u021bia de la idee la rezultat \u2013 \u0219i de la experiment la impact \u0219tiin\u021bific<\/p>\n","protected":false},"excerpt":{"rendered":"

    Platforma de Cercetare \u00een Biologie \u0219i Ecologie Sistemic\u0103 (PCBE), apar\u021bin\u00e2nd Facult\u0103\u021bii de Biologie, Universitatea din Bucure\u0219ti, reune\u0219te pe mai multe etaje o infrastructur\u0103 modern\u0103 pentru cercetare fundamental\u0103 \u0219i aplicat\u0103 \u2013 de la biologie molecular\u0103 \u0219i genomic\u0103, p\u00e2n\u0103 la culturi celulare, imagistic\u0103 avansat\u0103, citometrie \u00een flux, secven\u021biere de nou\u0103 genera\u021bie \u0219i microbiologie. PCBE este g\u00e2ndit\u0103 ca […]<\/p>\n","protected":false},"author":5,"featured_media":14137,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center 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